Detection of Viral Contamination in Animal Cell Cultures

Viral Contamination in Animal cell cultures

In cell lines, the natural products like serum in medium, and enzymes like trypsin, which is used for sub-culturing cell lines, are potential source of viral contamination. After continuous researches and studies it is possible that the bigger viruses can be removed from cultures during processing, but currently there is no proper and standard way to eliminate viral contamination completely. One way to avoid viral contamination is to make sure that the products sources like the animals are free from known viral diseases and infections.

Detection of viral contamination

Enzyme Linked Immunosorbent Assay (ELISA) is currently the most reliable method for detection of virus infection. Alternate way is by using Polymerase Chain Reaction (PCR) with correct viral primers.

Eradication of Viral Contamination

No such methods are currently available on which we can rely for elimination or removal of viruses from a culture. The only available options are tolerance or disposal.


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Did you read ? 

Detection of Mycoplasma Contamination in Animal Cell Cultures.

Also read: 

Maintenance of Sterility in Animal Tissue Culture.
Prevention of Cross-contamination in Animal cell cultures